Figure 1

Figure 1

Screening a hemofiltrate library for activity against extracellular Mycobacterium tuberculosis (Mtb). (A) Around 48 fractions (each 10 μl) of pool seven of the hemofiltrate library were incubated for 96 h with extracellular Mtb. ³H-Uracil was added for the final 24 h of incubation. The uptake of ³H-Uracil was measured by scintillation counting in a ß-counter. Antimicrobial activity was calculated by comparison to the untreated control. The bars present the average antimycobacterial activity (%) calculated from triplicates of a single experiment. (B) Subfractions 7–16/17 were analyzed via mass spectrometry. Spectrum analysis yielded three distinct peaks, identifying Angiogenin with a molecular mass of 14,137 Dalton. (C) Angiogenin sequence coverage (88%) by MS/MS analysis of the sample after carbamidomethylation and digestion with trypsin. Full sequence (Angiogenin precursor): signal peptide: 1–24 and Angiogenin 25–147.