Figure 9

Uptake of Angie1 and Angie1-lip in macrophages. (A) Macrophages were incubated overnight with Atto647N-labeled Angie1 or Angie1-lip (both 54 ?M, PSL Heidelberg). After 18 h, cells were harvested and analyzed by flow cytometry. Dot plots show one representative donor of three for each group. (B) The graph shows the mean fluorescence intensity (FI) ± SD of Atto647-positive cells for empty liposomes, Angie1 and Angie1-Lip of three independent donors. Statistical analysis was performed using a non-parametric Wilcoxon-Rank Test for paired samples. (C) The histogram shows the counts of Atto647-positive populations for empty liposomes, Angie1 and Angie1-Lip for one representative donor of three donors. (D) Macrophages were incubated with Angie1-Atto647N (upper panels) or Angie1-Atto647N-Lip (lower panels). After 18 h, cells were stained for MHC class II. Cell nuclei were stained with DAPI. Images were acquired using an inverted laser scanning confocal microscope (Zeiss LSM 710). Depicted images show representative area of one out of three donors with similar result.