Fig. 8

Mm burden was decreased by Hif‐1α stabilisation, despite delayed resolution of neutrophilic inflammation. (A) Schematic of experiment for B‐D. (B) Neutrophil numbers recruited to the tailfin wound at 6 hpw in mpx:GFP embryos. Groups are PR and DA Hif‐1α (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62–111 accumulated from three independent experiments. Statistics were determined using one‐way ANOVA (with Bonferroni’s multiple comparisons test). P values shown are as follows: ns = not significant and **P < 0.01. (C) Neutrophil numbers at the tailfin wound at 24 hpw. Groups are PR and DA Hif‐1α (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62–111 accumulated from three independent experiments. Statistics were determined using one‐way ANOVA (with Bonferroni’s multiple comparisons test). P values shown are as follows: ***P < 0.001. (D) Percentage resolution of neutrophil inflammation (between 6 and 24 hpw). Groups are PR and DA Hif‐1α (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62–111 accumulated from three independent experiments. Statistics were determined using one‐way ANOVA (with Bonferroni’s multiple comparisons test). P values shown are as follows: ns = not significant and **P < 0.01. (E) Schematic of experiment for G‐F. (F) Stereo‐fluorescence micrographs of Mm mCherry‐infected 4 dpi larvae after injection with DA Hif‐1α (DA1) and PR as a negative control and either wounded at 48 hpf or nonwounded in mpx:GFP embryos. Representative images from data shown in G, with n = 58 accumulated from three independent experiments. (G) Bacterial burden of larvae shown in (F). Data shown are mean ± SEM, n = 58 accumulated from three independent experiments. Statistics were determined using one‐way ANOVA (with Bonferroni’s multiple comparisons test). P values shown are as follows: ns = not significant, **P < 0.01, and ***P < 0.001.