Fig. 8

Mm burden was decreased by Hif?1? stabilisation, despite delayed resolution of neutrophilic inflammation. (A) Schematic of experiment for B?D. (B) Neutrophil numbers recruited to the tailfin wound at 6 hpw in mpx:GFP embryos. Groups are PR and DA Hif?1? (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62?111 accumulated from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ns = not significant and **P < 0.01. (C) Neutrophil numbers at the tailfin wound at 24 hpw. Groups are PR and DA Hif?1? (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62?111 accumulated from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ***P < 0.001. (D) Percentage resolution of neutrophil inflammation (between 6 and 24 hpw). Groups are PR and DA Hif?1? (DA) injected at 30 hpf with PVP or Mm. Data shown are mean ± SEM, n = 62?111 accumulated from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ns = not significant and **P < 0.01. (E) Schematic of experiment for G?F. (F) Stereo?fluorescence micrographs of Mm mCherry?infected 4 dpi larvae after injection with DA Hif?1? (DA1) and PR as a negative control and either wounded at 48 hpf or nonwounded in mpx:GFP embryos. Representative images from data shown in G, with n = 58 accumulated from three independent experiments. (G) Bacterial burden of larvae shown in (F). Data shown are mean ± SEM, n = 58 accumulated from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ns = not significant, **P < 0.01, and ***P < 0.001.