Fig. 1

Mm infection induced neutrophil emergency haematopoiesis and was increased by a comorbid wound. (A) Schematic of experiment for B, C and E. (B) Neutrophil numbers at the wound at 6 and 24 hpw of mpx:GFP embryos. Groups are NI, control injection with PVP and Mm injection. Data shown are mean ± SEM, n = 75?85 accumulated from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ns = not significant and ***P < 0.001. (C) Percentage resolution of neutrophilic inflammation (percentage change in neutrophil number at the tailfin wound between 6 and 24 hpw). Groups are NI, control injection with PVP and Mm injection. Data shown are mean ± SEM, n = 75?85 from three independent experiments. Statistics were determined using one?way ANOVA (with Bonferroni?s multiple comparisons test). P values shown are as follows: ns = not significant. (D) Total, whole?body neutrophil numbers at 2 dpf, after 18 hpi with PVP or Mm. Data shown are mean ± SEM, n = 69?74 accumulated from three independent experiments. Statistics were determined using an unpaired t?test. P values shown are as follows: ***P < 0.001. (E) Bacterial burden of larvae with or without a tailfin wound. Data shown are mean ± SEM, n = 58 accumulated from three independent experiments. Statistics were determined using an unpaired t?test. P values shown are as follows: ***P < 0.001.