Fig. 2

Local somite Mm infection lowers neutrophil numbers at the tailfin wound. (A) Schematic of experiment for B?E. (B) Total, whole?body neutrophil numbers in mpx:GFP embryos at 4 hpw after local somite injection with PVP or Mm. Data shown are mean ± SEM, n = 30?35 accumulated from three independent experiments. P values shown are as follows: ns = not significant. (C) Number of neutrophils at site of infection and tailfin wound at 4 hpi/w in mpx:GFP embryos. The groups are Wound (tailfin wound alone), Mm infection (Mm infection alone), Mock infection (PVP)/wound (injection of PVP into the somite alongside a tailfin wound) and Mm infection/wound (injection of Mm into the somite alongside a tailfin wound). Individual embryos are represented as lines joining their respective infection and tailfin wound neutrophil numbers. Data shown are mean ± SEM, n = 9?13 representative of three independent experiments. (D) Neutrophil numbers at the site of infection at 4 hpi. Data shown are mean ± SEM, n = 9?13 representative of three independent experiments. Statistics were determined using an unpaired t?test. P values shown are as follows: *P < 0.05, and **P < 0.01. (E) Neutrophil numbers at the site of tailfin wound at 4 hpi. Data shown are mean ± SEM, n = 9?13 representative of three independent experiments. Statistics were determined using an unpaired t?test. P values shown are as follows: *P < 0.05, and ***P < 0.001.