ZFIN ID: ZDB-FIG-210128-31
Petratou et al., 2021 - The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor. PLoS One   16:e0244794 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig 8 Mitfa represses <italic>tfec</italic> expression in melanoblasts.

In WT embryos at 24 hpf (A) and at 30 hpf (B), tfec expression is detectable in Ib(sp) and Ib(df) (arrows), respectively, and in the posteriorly regressing early NCC/Cbl domain of the dorsal posterior trunk and tail (region within arrowheads), but expression is undetectable in the lateral migration pathway. At 24 hpf (C) and at 30 hpf (D), mitfa mutants present with an increased number of tfec-positive cells (arrows) along the dorsal trunk, as well as in the medial and lateral (C, inset) migratory pathways. RNAscope (E,F) performed on mitfa mutants at 30 hpf shows an increased number of tfec-positive cells compared to the WT on the migration pathways (F, asterisks). Arrows indicate iridoblast precursors along the dorsal trunk. (F’) RNAscope reveals that in mitfa mutants ectopic tfec-positive cells migrating on the lateral migration pathway (below the epidermis; grey and yellow dashed lines indicate the periphery and nuclear boundary, respectively, of overlying keratinocytes) co-express ltk (arrows; nuclei indicated by purple dashed lines), and thus likely correspond to Ib(sp). ICM, intermediate cell mass; LP, lateral patches; no, notochord. Lateral views, head towards the left. Scale bars: A-D: 100 μm; E,F: 50 μm; F’: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ltk mitfaw2/w2 standard conditions Prim-15 iridoblast ISH
tfec WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 iridoblast ISH
Prim-5 post-vent region dorsal region ISH
Prim-5 trunk dorsal region ISH
Prim-15 intermediate cell mass of mesoderm ISH
Prim-15 iridoblast ISH
Prim-15 post-vent region dorsal region ISH
Prim-15 trunk dorsal region ISH
mitfaw2/w2 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 trunk dorsal region ISH
Prim-15 intermediate cell mass of mesoderm ISH
Prim-15 iridoblast ISH
Prim-15 trunk dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mitfaw2/w2 standard conditions Prim-5 trunk dorsal region tfec expression increased amount, abnormal
Prim-15 trunk dorsal region tfec expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One