ZFIN ID: ZDB-FIG-210128-25
Petratou et al., 2021 - The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor. PLoS One   16:e0244794 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 4

Fig 4 <italic>tfec</italic> can drive ectopic melanogenesis, and functions in the early stages of melanocyte development.

(A,B) tfec cDNA expressed from the ubiquitin promoter induces ectopic melanisation in wild-type embryos by 24 hpf. (C-F) Shown at 72 hpf, tfec expressed from either the ubi or sox10 promoters (E,F) rescues large well-differentiated melanocytes (black arrows) in mitfaw2/w2 embryos, in a manner reminiscent of sox10-driven mitfa (D), as well as apparently smaller, poorly melanised cells (blue arrows) (E,F). (G-I) Pigment cell phenotypes at 30 hpf. Compared to WT siblings (G), tfec mutants (H) have reduced melanisation of the RPE (arrowheads), and reduced melanocytes both along the dorsal trunk (arrows) and on the migratory pathways (asterisks). (I) Quantitation of melanocytes along the dorsal trunk and migratory pathways at 30 hpf reveals a 60% reduction in both regions in tfec mutants with respect to WT siblings. (J-L) Pigment cell phenotypes at 4 dpf. In embryos treated with melanin-concentrating hormone (MCH) to facilitate their quantitation, the number of trunk and tail melanocytes is not significantly altered along the dorsal, ventral or lateral stripes (J,K, red region; quantitated in L), however there is a statistically significant increase in the number of melanocytes located on the dorsal head (J,K, green region; quantitated in L). Scale bars: (A-F), 250 μm; (G,H,J,K), 200 μm. (I,L): spots signify outlier values; *, p-value < 10−9 using t-test.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mitfaw2/w2 standard conditions Prim-5 whole organism melanocyte absent, abnormal
Prim-15 whole organism melanocyte absent, abnormal
Protruding-mouth whole organism melanocyte absent, abnormal
Day 4 dorsal larval melanophore stripe melanocyte normal amount, normal
Day 4 head dorsal region increased accumulation melanocyte, abnormal
Day 4 lateral larval melanophore stripe melanocyte normal amount, normal
Day 4 ventral larval melanophore stripe melanocyte normal amount, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One