ZFIN ID: ZDB-FIG-210128-23
Petratou et al., 2021 - The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor. PLoS One   16:e0244794 Full text @ PLoS One
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig 2 <italic>tfec</italic> is a marker of differentiated iridophores, but not xanthophores.

(A–D) Chromogenic whole-mount in situ hybridisation reveals tfec expression in iridophores at 3 dpf. Iridophores (asterisks) of the posterior trunk/anterior tail are imaged live with reflected light in lateral (A) and dorsal (B) views. (C,D) Whole-mount in situ hybridisation on the same embryo reveals a pattern of tfec expression matching that of the differentiated iridophores (asterisks). The colour of the asterisks indicates corresponding iridophore patches between A and C, and B and D. Note the absence of expression in the location of the associated melanocytes (residual melanin indicated by arrowheads) (E-G). Expression of tfec (E, in situ hybridisation) and of Pax7 (F, immunofluorescence) in differentiated iridophores and xanthophores, respectively, at 48 hpf; note the absence of co-expression of these markers. A,C,E: lateral views; B,D: dorsal views. Head towards the left. Scale bars: A-D, 50 μm; E-G, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tfec WT standard conditions Long-pec iridophore ISH
Protruding-mouth iridophore ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-Pax7 IHC WT standard conditions Long-pec xanthophore
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One