FIGURE

Figure 7

ID

ZDB-FIG-210128-120

Publication

Fish et al., 2021 - Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders

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Figure 7

TTO measurements in fga morpholino knockdown embryos with transgenic expression of A?E, A?E R28C, or both, to model dysfibrinogenemia. (A) A scheme representing the experimental set-up. Early TU zebrafish embryos were injected with and without an fga mRNA-targeting morpholino (MO) in the absence or presence of Tol2 plasmids for expression of fibrinogen A?E, A?E R28C, A?E + A?E R28C or a half quantity of A?E (1/2 A?E). Tol2 transposase mRNA was also present within injection mixes (not shown). At 3 dpf the TTO assay was performed to measure venous thrombosis time. The MO inhibits endogenous fga mRNA splicing but cannot target the transgenically expressed A?E and A?E R28C mRNAs. (B) TTO data for each experimental group, each circle represents one larva (n = 19 to 22).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-fga
Condition:	light damage: posterior caudal vein
Knockdown Reagent:	MO1-fga
Observed In:	posterior caudal vein blood coagulation, fibrin clot formation posterior caudal vein platelet aggregation
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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