Figure 5.

Effects of sodium butyrate on caudal fin regeneration of adult zebrafish. (A) Images show Alcian blue and alizarin red staining of the regenerated fin in the control and SB (200 and 500 µM)-treated zebrafish at 14 dpa. Arrowheads, the amputation site. Scale bar, 2 mm. (B) Bars indicate the regeneration ratio of length, segments, and bifurcating ray at 14 dpa. Decreased regeneration rate was shown in only 500 µM SB-treated zebrafish. Data were expressed as the means ± S.E.M (n = 3). * p < 0.05 compared to the control. (C) Bars indicate the expression levels of wnt3a and lef1 mRNA in 500 µM SB-treated zebrafish fin at 2 dpa compared to control. Expression levels of lef1 mRNA increased in both control and SB-treated zebrafish at 2 dpa. The experiments were repeated 3 times. Data were expressed as the means ± S.E.M and were analyzed with Student’s t-tests. * p < 0.05 compared to the unamputated fin.