FIGURE

Fig. 2

ID

ZDB-FIG-201202-8

Publication

Loontiens et al., 2020 - PHF6 Expression Levels Impact Human Hematopoietic Stem Cell Differentiation

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Fig. 2

PHF6 is essential for normal hematopoietic differentiation. (A–D) (up) Dot plots show flow cytometry analysis of control and PHF6 shRNA transduced cord blood CD34⁺Lin^– precursors in OP9-GFP cocultures, showing the development of (A) CD19⁺HLA-DR⁺ B-lineage cells after 28 days of coculture, (B) CD56 + CD5- NK cells after 21 days of coculture, (C) CD14⁺ CD4⁺ monocytes after 14 days of coculture and (D) CD45⁺ CD71^– erythrocytes after 7 days of coculture. Bar plots (down) show absolute numbers of corresponding populations. (E) (up) Dot plots show flow cytometry analysis of control and PHF6 shRNA transduced cord blood CD34⁺Lin^– precursors in OP9-DLL1 cocultures, showing the development of CD34⁺CD7⁺ T cell precursors after 7 days of coculture. Bar plot (down) shows absolute numbers of the corresponding population. Data shows average of 5–7 independent experiments and error bars indicate SEM. *P < 0.05 (non-parametric paired Wilcoxon test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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