ZFIN Image: Loontiens et al., 2020, Fig. 2

Image

Figure Caption

Fig. 2

PHF6 is essential for normal hematopoietic differentiation. (A–D) (up) Dot plots show flow cytometry analysis of control and PHF6 shRNA transduced cord blood CD34⁺Lin^– precursors in OP9-GFP cocultures, showing the development of (A) CD19⁺HLA-DR⁺ B-lineage cells after 28 days of coculture, (B) CD56 + CD5- NK cells after 21 days of coculture, (C) CD14⁺ CD4⁺ monocytes after 14 days of coculture and (D) CD45⁺ CD71^– erythrocytes after 7 days of coculture. Bar plots (down) show absolute numbers of corresponding populations. (E) (up) Dot plots show flow cytometry analysis of control and PHF6 shRNA transduced cord blood CD34⁺Lin^– precursors in OP9-DLL1 cocultures, showing the development of CD34⁺CD7⁺ T cell precursors after 7 days of coculture. Bar plot (down) shows absolute numbers of the corresponding population. Data shows average of 5–7 independent experiments and error bars indicate SEM. *P < 0.05 (non-parametric paired Wilcoxon test).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol