Figure 1—figure supplement 2.
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- ZDB-FIG-201021-3
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- Helker et al., 2020 - Apelin signaling drives vascular endothelial cells towards a pro-angiogenic state
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BAC engineering was used to generate the apln:EGFP and aplnrb:aplnrb-TagRFP-sfGFP constructs. The clone RP71-2G21 was engineered to replace the ATG of apln with an EGFP cassette. The clone CH211-102K8 was engineered to replace the stop codon of aplnrb with a TagRFP-sfGFP cassette. To enhance the frequency of insertion into the genome, tol2 arms were added to the backbone of the BAC.
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