IMAGE

Figure 1—figure supplement 2.

ID: ZDB-IMAGE-201021-4
Publication: Helker et al., 2020 - Apelin signaling drives vascular endothelial cells towards a pro-angiogenic state
All Figures: Figures for Helker et al., 2020

Image

Figure Caption

Figure 1—figure supplement 2.

Generation of the Tg^BAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.

BAC engineering was used to generate the apln:EGFP and aplnrb:aplnrb-TagRFP-sfGFP constructs. The clone RP71-2G21 was engineered to replace the ATG of apln with an EGFP cassette. The clone CH211-102K8 was engineered to replace the stop codon of aplnrb with a TagRFP-sfGFP cassette. To enhance the frequency of insertion into the genome, tol2 arms were added to the backbone of the BAC.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife

Figure 1—figure supplement 2.

Figure 1—figure supplement 2.

Generation of the TgBAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.

Generation of the Tg^BAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.