FIGURE

Figure 3

ID

ZDB-FIG-201003-258

Publication

Bedell et al., 2020 - A vertebrate model to reveal neural substrates underlying the transitions between conscious and unconscious states

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Ketamine hill plots and absorption. (A, B) Change in behavior for SPONT (blue), TAP (green) and ELEC (purple) required very high doses of ketamine at pH 7.2 (A) versus pH 8 (B). For the TAP (A, green), complete loss of movement was unable to be attained. Graph was created with a minimum of three replicates per point and 8–12 zebrafish larvae used per replicate. Ketamine at pH 8 (B) requires a fourfold higher concentration for TAP versus ELCT. (C) We used HPLC to determine the ketamine concentration in the zebrafish larvae over time. We found a significantly higher concentration of ketamine at pH 8 versus pH 7.2. Additionally, this shows that the ketamine equilibrated at approximately 30 min. Each point was replicated five times and contained 20–25 larval zebrafish per replicate.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.