Figure 1

Zebrafish movement before and after TAP and ELEC. (A) The zebrafish were behaviorally equilibrated within the chambers used to induce stimuli. Prior to a stimulus (1) the distance moved over 2 s was stable in both chambers, which the circular tap wells having a higher average distance moved when compared to the rectangular ELEC chamber. At the 0 time point, the zebrafish larvae were stimulated with either tap or electrical shock. The following 36–40 s (block box magnification) the zebrafish with the TAP (green) moved normally whereas the ELEC demonstrated a decrease in movement (purple). (B) When averaging the 2 s of distance moved for the 120 s prior to the stimulus (1), the 40 s immediately following the stimulus (2) and 40–90 s after the stimulus, there is no significant difference in movement for the TAP (green). However, for the ELEC (purple), there is a statistically significant decrease in movement following the stimulus but the larvae recover back to baseline movement after approximately 40 s. Five replicates where assessed with 8–12 larvae per replicate.