ZFIN Image: Bedell et al., 2020, Figure 3

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Figure Caption

Figure 3

Ketamine hill plots and absorption. (A, B) Change in behavior for SPONT (blue), TAP (green) and ELEC (purple) required very high doses of ketamine at pH 7.2 (A) versus pH 8 (B). For the TAP (A, green), complete loss of movement was unable to be attained. Graph was created with a minimum of three replicates per point and 8–12 zebrafish larvae used per replicate. Ketamine at pH 8 (B) requires a fourfold higher concentration for TAP versus ELCT. (C) We used HPLC to determine the ketamine concentration in the zebrafish larvae over time. We found a significantly higher concentration of ketamine at pH 8 versus pH 7.2. Additionally, this shows that the ketamine equilibrated at approximately 30 min. Each point was replicated five times and contained 20–25 larval zebrafish per replicate.

Acknowledgments

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