ZFIN ID: ZDB-FIG-201003-186
Houseright et al., 2020 - Cell type specific gene expression profiling reveals a role for complement component C3 in neutrophil responses to tissue damage. Scientific Reports   10:15716 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Figure 2

TRAP-RNAseq identifies upregulation of the complement pathway and c3a.1 in response to wounding. (A) Expression measured by RNA-seq (fpkm) of complement pathway gene c3a.1 across all three cell types. Each dot represents one replicate. (B) RT-qPCR validation of c3a.1 gene expression in neutrophils and macrophages. Each dot represents one replicate; measurements for each replicate were performed in triplicate. Data is expressed as the fold change in mean Cq, normalized to ef1a expression and expression of c3a.1 in unwounded controls. N = 200 larvae/condition/replicate. C, D. Expression measured by RNA-seq (fpkm) of complement pathway genes c5 (C) and c9 (D), across all 3 three cell types. Each dot represents one replicate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
c3a.1 uwm15Tg standard conditions Protruding-mouth neutrophil RNA-Seq
Protruding-mouth neutrophil RTPCR
uwm15Tg transection: caudal fin Protruding-mouth neutrophil RTPCR
Protruding-mouth neutrophil RNA-Seq
uwm16Tg standard conditions Protruding-mouth macrophage RTPCR
Protruding-mouth macrophage RNA-Seq
uwm16Tg transection: caudal fin Protruding-mouth macrophage RTPCR
Protruding-mouth macrophage RNA-Seq
uwm17Tg standard conditions Protruding-mouth epithelial cell RNA-Seq
uwm17Tg transection: caudal fin Protruding-mouth epithelial cell RNA-Seq
c5 uwm15Tg standard conditions Protruding-mouth neutrophil RNA-Seq
uwm15Tg transection: caudal fin Protruding-mouth neutrophil RNA-Seq
uwm16Tg standard conditions Protruding-mouth macrophage RNA-Seq
uwm16Tg transection: caudal fin Protruding-mouth macrophage RNA-Seq
uwm17Tg standard conditions Protruding-mouth epithelial cell RNA-Seq
uwm17Tg transection: caudal fin Protruding-mouth epithelial cell RNA-Seq
c9 uwm15Tg standard conditions Protruding-mouth neutrophil RNA-Seq
uwm15Tg transection: caudal fin Protruding-mouth neutrophil RNA-Seq
uwm16Tg standard conditions Protruding-mouth macrophage RNA-Seq
uwm16Tg transection: caudal fin Protruding-mouth macrophage RNA-Seq
uwm17Tg standard conditions Protruding-mouth epithelial cell RNA-Seq
uwm17Tg transection: caudal fin Protruding-mouth epithelial cell RNA-Seq
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
uwm15Tg transection: caudal fin Protruding-mouth neutrophil c3a.1 expression increased amount, abnormal
Protruding-mouth neutrophil c3a.1 expression increased amount, abnormal
uwm16Tg transection: caudal fin Protruding-mouth macrophage c3a.1 expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.