Figure 5

Loss of c3a.1 impairs neutrophil recruitment by decreasing neutrophil migration speed early after wounding. (A) Time-lapse photomicrographs of neutrophil recruitment to tail-transected caudal fins of c3a.1^+/+ (n = 8) or c3a.1^−/− larvae (n = 8) with mcherry-labeled neutrophils (Tg(mpx:mcherry)), 0–60 min post-wound, showing tracks of forward migrating neutrophils. Images with track overlay were generated using the MTrackJ plugin⁶⁸ in ImageJ⁶⁹ (B) Quantification of mean track speed of forward-migrating neutrophils, expressed as mean + /- SEM. Each dot represents the mean of the first 5 neutrophils recruited to the wound of an individual larva. Colors represent the results of 4 independent experiments. *p < 0.05 (C) Graph, expressed as mean, and (D) quantification of instantaneous speed of all forward-migrating neutrophils over the first 60 min following wounding. In (D), for speed and fold change, data are expressed as median (center values), with 95% confidence intervals (small print). Data comprise 4 independent experiments. *p < 0.05.