FIGURE

Figure 6—figure supplement 1.

ID

ZDB-FIG-201003-177

Publication

Boezio et al., 2020 - Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract

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Figure 6—figure supplement 1.

Alk5 regulates the expression of ECM genes in the cardiac OFT, including that of <italic>fbln5.</italic>

(A) Schematic showing the setting for the RNA-seq on extracted embryonic hearts (56 hpf). (B) Gene ontology bar plot, showing the most overrepresented cellular compartments up- or down-regulated in the Alk5 inhibitor-treated embryos compared with DMSO-treated ones. (C) Gene ontology bar plot, showing some of the most overrepresented biological processes downregulated in the Alk5 inhibitor-treated embryos compared to DMSO-treated ones. (D) Venn diagram depicting the genes downregulated (log2FC < −0.585) in the Alk5 inhibitor-treated embryonic hearts, exclusively expressed in the adult zebrafish BA (Singh et al., 2016), and part of the zebrafish matrisome (Nauroy et al., 2018). The five genes in the intersection are listed in the box. (E) Relative mRNA levels of the five genes in the intersection obtained from 72 hpf alk5+/+ and alk5-/- larval hearts. Means ± SD; p values from Mann Whitney tests; average Ct values are listed in Supplementary file 2. (F) MA plot displaying differentially expressed genes between Alk5 inhibitor-treated and control animals. fbln5 is highlighted among the downregulated genes (red).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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