FIGURE

Fig. 5

ID

ZDB-FIG-200910-38

Publication

Chetty et al., 2020 - Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Ets1 exhibits delayed activity in inducing endothelial gene expression compared to Etv2. (A–C) Compound microscope images of GFP fluorescence in Tg(fli1a:GFP) wild-type, etv2 mRNA and ets1 mRNA-injected embryos at the tailbud (10 hpf) stage. Note the GFP fluorescence in etv2 mRNA-injected embryos (B) and the lack thereof in ets1 mRNA-injected embryos (C). (D–I) GFP fluorescence in Tg(fli1a:GFP) wild-type, XeX:etv2 and XeX:ets1-injected embryos at the tailbud and 20-somite stage (19 hpf). Note the absence of GFP fluorescence in XeX:ets1 embryos at the tailbud stage (F) but presence of ectopic GFP expression at 20-somite stage as indicated by white arrowheads (I). (J–L) GFP fluorescence in Tg(fli1a:GFP) wild-type, etv2 MO and etv2 MO; XeX:ets1 embryos at 18-somite stage (18 hpf). Note the GFP expression induced by XeX:ets1 even in the absence of Etv2 function (L). (M) qPCR of wildtype, XeX:etv2 and XeX:ets1 whole embryos at the tailbud and 10-somite stage. ∗P < 0.05, ∗∗P < 0.01 (P value was calculated between wt and injected embryos); n.s – not significant; error bars represent ± SEM.

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Anatomical Term:	endothelial cell
Stage Range:	Bud to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Observed In:	endothelial cell
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.