Fig. 5

Fig. 5 Ets1 exhibits delayed activity in inducing endothelial gene expression compared to Etv2. (A–C) Compound microscope images of GFP fluorescence in Tg(fli1a:GFP) wild-type, etv2 mRNA and ets1 mRNA-injected embryos at the tailbud (10 hpf) stage. Note the GFP fluorescence in etv2 mRNA-injected embryos (B) and the lack thereof in ets1 mRNA-injected embryos (C). (D–I) GFP fluorescence in Tg(fli1a:GFP) wild-type, XeX:etv2 and XeX:ets1-injected embryos at the tailbud and 20-somite stage (19 hpf). Note the absence of GFP fluorescence in XeX:ets1 embryos at the tailbud stage (F) but presence of ectopic GFP expression at 20-somite stage as indicated by white arrowheads (I). (J–L) GFP fluorescence in Tg(fli1a:GFP) wild-type, etv2 MO and etv2 MO; XeX:ets1 embryos at 18-somite stage (18 hpf). Note the GFP expression induced by XeX:ets1 even in the absence of Etv2 function (L). (M) qPCR of wildtype, XeX:etv2 and XeX:ets1 whole embryos at the tailbud and 10-somite stage. ∗P ​< ​0.05, ∗∗P ​< ​0.01 (P value was calculated between wt and injected embryos); n.s – not significant; error bars represent ​± ​SEM.