FIGURE

Fig. 1

ID

ZDB-FIG-200910-34

Publication

Chetty et al., 2020 - Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish

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Fig. 1

Loss of Ets1 function exacerbates vascular defects in etv2?/?embryos. (A-H?) Confocal images of Tg(kdrl:GFP) expression in wt, ets1?/?, etv2?/?and ets1?/?; etv2?/?embryos at 34 hpf and 60 hpf using 4� and 10� objectives. ets1?/?embryos display normal vascular patterning (B-B?, F-F?), similar to wild type embryos (A-A?,E-E?). ISVs begin to emerge in etv2?/?embryos at ~34 hpf (C?) but are absent in ets1?/?; etv2?/? double mutant embryos (D?). At 60 hpf, ets1?/?; etv2?/?embryos have fewer ISVs and less recovery in axial vasculature than etv2?/? embryos (G-H?). (I?J) Quantitative analysis of total number of ISVs and ISV height at 60 hpf. Note the reduction in number of ISVs (I) and full sprouts (J) in ets1?/?; etv2?/?compared to etv2?/?embryos. ?P ?< ?0.05, ??P ?< ?0.01; n.s ? not significant. Horizontal bars within violin plots represent median. Arrowheads indicate mis-patterned and stunted ISVs.

Expression Data

Gene:	EGFP
Fish:	s843Tg ets1^ci14/ci14; s843Tg etsrp^y11/y11; s843Tg ets1^ci14/ci14; etsrp^y11/y11; s843Tg
Anatomical Terms:	blood vasculature blood vessel intersegmental vessel
Stage Range:	Prim-15 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ets1^ci14/ci14; s843Tg etsrp^y11/y11; s843Tg ets1^ci14/ci14; etsrp^y11/y11; s843Tg
Observed In:	blood vasculature blood vessel intersegmental vessel intersegmental vessel vascular sprouts
Stage Range:	Prim-15 to Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.