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Effect of β-casomorphins on β-cell regeneration. (A) β-cell ablation in Tg (ins: CFP-NTR was performed using 12 mM MTZ from 3–4 dpf. No positively fluorescent cells were detected at 4 dpf indicating complete ablation (black arrowheads indicate β-cells). (B) Tg (ins: CFP-NTR) zebrafish embryos were treated with 12 mM MTZ from 3–4 dpf to ablate the pancreatic β and exposed to daily 24 h treatments of bBCM-5, bBCM-7, hBCM-5 and hBCM-7 from 4–7 dpf to analyse the effects of β-casomorphins on β-cell regeneration (black arrowheads indicate β-cells). (C) Statistical analysis using Student's t-test revealed a significant decrease in the area of CFP fluorescence (measured using Fiji) following treatment with bBCM-5 and bBCM-7 compared to ablated controls at 7 dpf. No changes were detected in the area of CFP fluorescence following treatment with hBCM-5 and hBCM-7 compared to ablated controls at 7 dpf. Data represent mean±s.e.m.; *P<0.0005; Student's t-test. (n=20).
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