PUBLICATION
The protective effects of human milk derived peptides on the pancreatic islet biology
- Authors
- Singh, A., Enjapoori, A.K., Gibert, Y., Dwyer, K.M.
- ID
- ZDB-PUB-200724-4
- Date
- 2020
- Source
- Biology Open 9(8): (Journal)
- Registered Authors
- Gibert, Yann
- Keywords
- Beta cell, Bovine beta casomorphin, Human beta casomorphin, Pancreas, Regeneration, Type 1 diabetes, Zebrafish
- MeSH Terms
-
- Animals
- Cattle
- Endorphins/pharmacology
- Glucagon/metabolism
- Humans
- Insulin/metabolism
- Islets of Langerhans/drug effects
- Islets of Langerhans/physiology*
- Milk, Human/chemistry*
- Peptides/pharmacology*
- Receptors, Opioid, mu/metabolism
- Regeneration/drug effects
- Somatostatin/metabolism
- Zebrafish
- PubMed
- 32694188 Full text @ Biol. Open
Citation
Singh, A., Enjapoori, A.K., Gibert, Y., Dwyer, K.M. (2020) The protective effects of human milk derived peptides on the pancreatic islet biology. Biology Open. 9(8):.
Abstract
Several epidemiological studies support the protective role of breast-feeding in reducing the risk for type 1 diabetes. Human breast milk is the perfect nutrition for infants and contains many complex proteins, lipids and carbohydrates. In this study, we examined the physiological effects of human milk-derived opioid peptides, beta casomorphins (BCM), and compared them with bovine milk-derived opioid peptides on pancreatic hormone regulation and β-cell regeneration. Exposure of wild-type zebrafish embryos to 50 µg/mL of human BCM -5 and -7 from 3 days post fertilisation until 6 days post fertilisation resulted in an increased insulin domain of expression while exposure to bovine BCM -5 and -7 significantly reduced the insulin domain of expression as analysed by whole-mount in-situ hybridisation. These changes may be accounted for by reduced insulin expression or β-cell number and were mitigated by the µ-opioid receptor antagonist, naloxone. The effect of BCM on β-cell regeneration was assessed following ablation of β-cells in Tg (ins: CFP-NTR) zebrafish from 3 days post fertilisation to 4 days post fertilisation, followed by exposure of bovine and human BCM -5 and -7 (50 µg/mL) from 4 days post fertilisation until 7 days post fertilisation. The regenerative capacity of β-cells was not impeded following exposure to human BCM -5 and -7, whereas the capacity of β-cells to regenerate following bovine BCM -5 and -7 exposure was reduced. Our data suggests that human BCM -5 and -7 may promote β-cell development and enable the regeneration of β-cells, while the bovine milk derived peptides, BCM -5 and -7 play an opposite role. These data may provide some biological explanation for the protective effect of breastfeeding on the development of Type 1 Diabetes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping