WISH to analyse the effect of chronic β-casomorphin exposure on glucagon and somatostatin domain of expression. (A) Glucagon (black arrowheads) and somatostatin (black arrows) domain of exposure in WT zebrafish embryos exposed to 50 µg/ml of bBCM-5, bBCM-7, hBCM-5 and hBCM-7 from 3–6 dpf. (B) Statistical analysis of glucagon and somatostatin domain of expression using Student's t-test. Fold change (compared to untreated control zebrafish embryos) shows glucagon domain of expression significantly increased following bBCM-7 exposure. No significant change in glucagon and somatostatin domain of exposure was detected following bBCM-5, hBCM-5 and -7 exposure. Data represent mean±s.e.m.; *P<0.05; Student's t-test (n=10).
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