FIGURE

Fig. 3

ID

ZDB-FIG-200814-12

Publication

Asselin et al., 2020 - Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Expression of h<italic>KIF21B</italic> missense variants induces abnormal neuronal migration.

a, c Coronal sections of E18.5 (a) or P2 (c) mouse cortices electroporated at E14.5 with NeuroD-IRES-GFP empty vector (1 µg/µL) or WT, p.Gln313Lys, p.Ile678Leu or p.Ala1001Thr NeuroD-hKIF21B or co-expressing WT-hKIF21B together with mutated hKIF21B constructs (ratio 1:1). GFP-positive electroporated cells are depicted in green. Nuclei are stained with DAPI. b, d Analysis (means ± S.E.M.) of the percentage of electroporated GFP-cells in different regions (Up CP: upper cortical plate, Lo CP: lower cortical plate, IZ: intermediate zone, SVZ: subventricular zone) showing effect of expressing hKIF21B variants. Data were analyzed by two-way ANOVA (Bonferroni’s multiple comparisons test). Number of embryos analyzed: b Empty vector, n = 6; WT, n = 13; p.Gln313Lys, n = 6; p.Ile678Leu, n = 11; p.Ala1001Thr, n = 8. Rescue experiments: n = 6 for each condition. d Empty vector, n = 3; WT, n = 4; p.Gln313Lys, n = 6; p.Ile678Leu, n = 6; p.Ala1001Thr, n = 4. ns non-significant; *P < 0.05; **P < 0.005; ***P < 0.001; ****P < 0.0001. e Cux1-immunolabeling (gray) of P2 coronal sections of mouse brains electroporated at E14.5 with the WT or p.Ile678Leu NeuroD-hKIF21B constructs showing no specification defects of arrested neurons (green). Scale bars (a, c, e) 50 μm. Source data are provided in the Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.