FIGURE

Fig. 5

ID

ZDB-FIG-200814-14

Publication

Asselin et al., 2020 - Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activity

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Fig. 5

Expression of p.Gln313Lys hKIF21B decreases head size in zebrafish larvae.

a Dorsal view of representative control zebrafish larvae (non-injected) or injected with 100 pg of wild-type (WT) or mutated hKIF21B mRNAs (p.Gln313Lys and p.Ala1001Thr) at 5 days post-fertilization (5 dpf). Double arrow indicates the distance between the forebrain and hindbrain, a measure used as a proxy for head size. b Dot plot of the head measurements (red double arrow) of control and injected larvae at 5 dpf. Red diamond corresponds to the mean of the batch measured. Number of embryos analyzed for this specific batch: control, n = 40; WT, n = 31; p.Gln313Lys, n = 38; p.Ala1001Thr, n = 31. Experiments were repeated six times for non-injected control embryos (n = 231), four times for WT-injected embryos (n = 127), three times for p.Gln313Lys-injected embryos (n = 108) and four times for p.Ala1001Thr-injected embryos (n = 158). ns, non-significant, *P < 0.05, **P < 0.005, ***P < 0.001. Significance was calculated by unpaired two-tailed Student’s t-test or a Welch’s two sample t-test between control and RNA-injected larvae. ns non-significant, *P < 0.05, **P < 0.005, ***P < 0.001. Source data are provided in the Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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