Fig. 3

Fig. 3

a, c Coronal sections of E18.5 (a) or P2 (c) mouse cortices electroporated at E14.5 with NeuroD-IRES-GFP empty vector (1 µg/µL) or WT, p.Gln313Lys, p.Ile678Leu or p.Ala1001Thr NeuroD-hKIF21B or co-expressing WT-hKIF21B together with mutated hKIF21B constructs (ratio 1:1). GFP-positive electroporated cells are depicted in green. Nuclei are stained with DAPI. b, d Analysis (means ± S.E.M.) of the percentage of electroporated GFP-cells in different regions (Up CP: upper cortical plate, Lo CP: lower cortical plate, IZ: intermediate zone, SVZ: subventricular zone) showing effect of expressing hKIF21B variants. Data were analyzed by two-way ANOVA (Bonferroni’s multiple comparisons test). Number of embryos analyzed: b Empty vector, n = 6; WT, n = 13; p.Gln313Lys, n = 6; p.Ile678Leu, n = 11; p.Ala1001Thr, n = 8. Rescue experiments: n = 6 for each condition. d Empty vector, n = 3; WT, n = 4; p.Gln313Lys, n = 6; p.Ile678Leu, n = 6; p.Ala1001Thr, n = 4. ns non-significant; *P < 0.05; **P < 0.005; ***P < 0.001; ****P < 0.0001. e Cux1-immunolabeling (gray) of P2 coronal sections of mouse brains electroporated at E14.5 with the WT or p.Ile678Leu NeuroD-hKIF21B constructs showing no specification defects of arrested neurons (green). Scale bars (a, c, e) 50 μm. Source data are provided in the Source Data file.