FIGURE

Fig. 4

ID

ZDB-FIG-200813-6

Publication

Lex et al., 2020 - Telomere shortening produces an inflammatory environment that increases tumor incidence in zebrafish

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Fig. 4

tert ?/? tissues present increased levels of senescence (p15/16) and inflammation (TNF-?). (A) Representative immunofluorescence images of p15/16 and TNF-? in the skin near melanoma in chimeric WT and tert?/? zebrafish. Dashed lines locate the skin (no green fluorescence), and arrows indicate p15/16-positive cells. Squares with dashed lines show place of amplification in a sequential section, and squares with solid lines show place of amplification on that section. (B and C) Quantification of p15/16-positive cells and levels of TNF-?, respectively, in the skin of chimeric WT and tert?/? zebrafish (n = 3). (D?F) RT-qPCR analysis comparing the expression levels of cdkn2a/b (p15/16), cdkn1a (p21), and tnfa (TNF) of 4-dpf WT, G2 tert?/? larvae, 9-months WT, and tert?/? adult intestine tissue. Data are represented as mean � SEM. (*P < 0.05; **P < 0.01 for n = 30).

Expression Data

Genes:	cdkn1a tnfa
Antibody:	Ab1-tnfa
Fish:	WT mitfa^w2/w2; tert^{hu3430/hu3430}; mpv17^a9/a9
Anatomical Term:	integument
Stage Range:	Day 4 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; tert^{hu3430/hu3430}; mpv17^a9/a9
Observed In:	inflammatory response integument whole organism
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

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