FIGURE

Fig. 5

ID

ZDB-FIG-200813-7

Publication

Lex et al., 2020 - Telomere shortening produces an inflammatory environment that increases tumor incidence in zebrafish

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Fig. 5

G2 tert mutant cells induce high levels of senescence and systemic inflammation in host larvae. (A) Representative images of SA-β-Gal assay comparing WT and G2 tert−/− 4-dpf zebrafish embryos. Yolk sack staining is nonspecific. (B, Left) Scheme of chimera generation: G2 tert−/− blastula cells are transplanted into WT embryos (G2 tert−/− → WT). (B, Right) Marked senescence in 4-dpf WT embryos upon G2 tert−/− cell injection (C, Left) Scheme of chimera generation: WT or G2 tert−/− blastula cells transplanted into WT Tg(mpx:GFP) embryos with GFP-labeled neutrophils: WT → WT Tg(mpx:GFP) vs. G2 tert−/− → WT Tg(mpx:GFP). (C, Right) Representative images at 4 dpf, and neutrophils are shown in green. (Scale bars: 0.5 mm.) (D) Quantification of neutrophils at 4 dpf. Noninjected Tg(mpx:GFP) were used as controls. Each data point represents one zebrafish (**P > 0.01; noninjected n = 24; WT n = 33; tert−/− n = 25; ns, nonsignificant [P > 0.05]).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA