alpl expression pattern (combined with PCNA IF) and Tnap-activity in sections of the adult mesencephalon, diencephalon, and hypothalamus. The cutting planes of the different sections were indicated in schematic overviews through black lines and marked with numbers. (A) Cross sections of adult zebrafish brain stained with alpl ISH (red) and nuclear staining using DAPI (blue) in the region of the mesencephalon, diencephalon, and hypothalamus. Scale bars: 50 µm (high resolution photos) or 100 µm (overview). The alpl ISH signals were detected, among others, in ventricular areas (DiV, TeV), the dorsomedial optic tract (DOT), and the hypothalamus (HC). (B) ELF 97-staining showed sites of AP-activity within the torus longitudinalis (TL), the hypothalamus (TH/IL), and the medulla oblongata (Mo). (C) Cross sections of adult zebrafish brain co-stained with alpl ISH (red), PCNA IF (green), and DAPI (blue) in the region of the mesencephalon. Scale bars: 50 µm or 100 µm. Merged alpl ISH, PCNA IF, and DAPI signals were shown. The respective localizations of the region of interests, depicted in the high resolution pictures, was indicated with white boxes. The alpl ISH signals were marked with a white arrows, PCNA signals with a green arrowheads, and cells that were positive for alpl and PCNA were indicated with yellow arrowheads. Bv, blood vessel; CC/CCe, cerebellum; Dc/Dd/Dl, central/dorsal/lateral zone of telencephalon; Dil, inferior lobe; DiV, diencephalic ventricle; DOT, dorsomedial optic tract; Hc/Hd, caudal/dorsal zone of periventricular hypothalamus; Mo, Medulla oblongata; TeV, telencephalic ventricles; TeO, Tectum opticum; TH/IL, hypothalamus; TL, torus longitudinalis; A/VM, anterior/ ventromedial thalamic nucleus; Val/Vam, valvula cerebelli.
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