ZFIN ID: ZDB-FIG-200724-25
Lee et al., 2020 - Human ARF Specifically Inhibits Epimorphic Regeneration in the Zebrafish Heart. Genes   11(6) Full text @ Genes (Basel)
Anatomical Term:
Stage: Adult
Observed In:
Stage: Adult

Figure 1

Induced Alternative Reading Frame (ARF) expression in hs:ARF fish suppresses cardiac regeneration. (a) Representative polymerase chain reaction (PCR) product of ARF expression from three experimental replicates of wild type (WT) and hs:ARF heart RNA on 15 dpi. The product run on gel electrophoresis shows ARF expression present in hs:ARF fish while absent in WT fish. (b) Acid Fuchsin Orange G (AFOG) and troponin staining of WT and hs:ARF heart cryosections on 15 days post-injury (dpi). The dotted lines demarcate the injury site. Red stains are for fibrin. Blue stains are for collagen. Brown stains are for muscle. Green stains are for troponin. (c) Myocardial recovery measured by troponin infiltration into the injury site. Infiltration quantified by imaging software shows decreased troponin in the injury site of hs:ARF fish compared to WT fish. N = 8 hearts. Results are shown as mean ± standard error. The * represents a statistically significant difference.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab7-tnnt IHC zf706Tg(AB) cold damage: heart, heat shock Adult heart regenerating tissue
Phenotype Details
Fish Conditions Stage Phenotype
zf706Tg(AB) cold damage: heart, heat shock Adult heart cardiac muscle tissue regeneration disrupted, abnormal
Adult heart regenerating tissue Ab7-tnnt labeling decreased amount, abnormal
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