FIGURE

Fig. 1.

ID

ZDB-FIG-200713-31

Publication

Peron et al., 2020 - The stem-like STAT3-responsive cells of zebrafish intestine are WNT/β-catenin dependent

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Fig. 1.

Generation of Tg(7xStat3-Hsv.Ul23:EGFP) fish and characterization of their expression pattern. (A) Scheme of the Tol-2 vectors used to generate the Tg(7xStat3-Hsv.Ul23:EGFP) reporter. (B,B′) Diffused EGFP is detectable in early stage embryos obtained by outcrossing transgenic females (B), not with transgenic males (B′). (C-F) At 22 hpf, EGFP expression is detectable in the anterior telencephalic region (t), the primordial midbrain hindbrain boundary (mhb), the hindbrain (h), the primitive neuromasts (n) and in the haematopoietic tissue (ht). (G,H) At 48 hpf, EGFP expression is mostly located in the optic tectum (TeO) and in the hindbrain (h). (I,J) Starting from 4 dpf, EGFP expression is detectable in the developing intestine in isolated pear-shaped cells (I); the intestinal fluorescence lasts throughout adulthood (J).

Expression Data

Gene:	EGFP
Fish:	ia28Tg ia29Tg
Anatomical Terms:	hindbrain intermediate cell mass of mesoderm intestinal epithelium midbrain midbrain hindbrain boundary optic tectum telencephalon
Stage Range:	26+ somites to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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