PUBLICATION

The stem-like STAT3-responsive cells of zebrafish intestine are WNT/β-catenin dependent

Authors
Peron, M., Dinarello, A., Meneghetti, G., Martorano, L., Facchinello, N., Vettori, A., Licciardello, G., Tiso, N., Argenton, F.
ID
ZDB-PUB-200530-6
Date
2020
Source
Development (Cambridge, England)   147(12): (Journal)
Registered Authors
Argenton, Francesco, Facchinello, Nicola, Tiso, Natascia, Vettori, Andrea
Keywords
Intestine, Stat3, Stem cells, Wnt/?-catenin, Zebrafish
MeSH Terms
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Animals
  • Wnt Signaling Pathway*/drug effects
  • Larva/growth & development
  • Larva/metabolism
  • Janus Kinase 1
  • SOX9 Transcription Factor/metabolism
  • Intestines/growth & development
  • Intestines/physiology
  • beta Catenin/metabolism
  • Protein Kinase Inhibitors/pharmacology
  • Transcription, Genetic/drug effects
  • Intestinal Mucosa/metabolism*
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Transcription Factor 7-Like 2 Protein/deficiency
  • Transcription Factor 7-Like 2 Protein/genetics
  • Transcription Factor 7-Like 2 Protein/metabolism
  • Tumor Suppressor Proteins/genetics
  • Tumor Suppressor Proteins/metabolism
  • STAT3 Transcription Factor/antagonists & inhibitors
  • STAT3 Transcription Factor/deficiency
  • STAT3 Transcription Factor/genetics
  • STAT3 Transcription Factor/metabolism*
  • Wnt Proteins/metabolism
(all 30)
PubMed
32467235 Full text @ Development
Abstract
The transcription factor STAT3 is required for proliferation and pluripotency of embryonic stem cells; we have prepared and characterized fluorescent STAT3-reporter zebrafish based on repeats of minimal responsive elements. These transgenic lines mimic in vivo STAT3 expression patterns and are responsive to exogenous STAT3; notably, fluorescence is inhibited by both stat3 knock-out and IL6/JAK/STAT inhibitors. At larval stages, STAT3 reporter activity correlates with proliferating regions of the brain, haematopoietic tissue and intestine. In the adult gut the reporter is active in sparse proliferating cells, located at the base of intestinal folds, expressing the stemness marker sox9b and having the mammalian Crypt Base Columnar cells morphology; noteworthy, zebrafish stat3 mutants show defects in intestinal folding. The STAT3 reporter activity in the gut is abolished in mutants of Tcf7l2, the intestinal mediator of Wnt/β-catenin-dependent transcription, and the Wnt/β-catenin dependence of STAT3 activity in the gut is confirmed by abrupt expansion of STAT3-positive cells in intestinal adenomas of apc heterozygotes. Our findings indicate that Jak/STAT3 signalling is needed for intestinal stem cells maintenance and possibly crucial in controlling Wnt/β-catenin-dependent colorectal cancer cells proliferation.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Peron, M., Dinarello, A., Meneghetti, G., Martorano, L., Facchinello, N., Vettori, A., Licciardello, G., Tiso, N., Argenton, F. (2020) The stem-like STAT3-responsive cells of zebrafish intestine are WNT/β-catenin dependent. Development (Cambridge, England). 147(12):.