FIGURE

Fig. 9

ID

ZDB-FIG-200629-25

Publication

Chen et al., 2020 - Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy

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Fig. 9

Hepatic accumulation of Chk1 and Wee1 in capn3b^Δ19Δ14 at 3dpH. a Elevation of p53 protein level in capn3b^Δ19Δ14 after PH. Western blot analysis of p53 at different time point after PH as indicated. Total proteins were extracted from the liver tissues of sham, 3dpH, 5dpH, 7dpH, 10dpH and 14dpH, respectively. GAPDH, loading control. b Western blot of Capn3b and Chk1 in WT and capn3b^Δ19Δ14 mutant embryos at 3.5dpf and 5dpf grown at 30 °C and 34.5 °C, respectively. The embryos were shifted to 34.5 °C at 12hpf till the time of harvesting. β-Actin: loading control. c Western blot of Chk1 in WT and capn3b^Δ19Δ14 sham and PH groups at 3dpH group. Fibrillarin: loading control. d Co-immunostaining of Wee1 and Fibrillarin in the liver of WT and capn3b^Δ19Δ14 mutant fish at 3dpH. DAPI: staining nuclei

Expression Data

Genes:	capn3b tp53
Antibodies:	Ab1-capn3b Ab1-wee1 Ab5-chek1
Fish:	AB capn3b^zju20/zju20 (AB)
Conditions:	heat exposure amputation: liver
Anatomical Terms:	liver liver nucleolus liver nucleus liver regenerating tissue whole organism
Stage Range:	Protruding-mouth to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB capn3b^zju20/zju20 (AB)
Conditions:	heat exposure amputation: liver
Observed In:	liver liver nucleolus liver nucleus liver regenerating tissue whole organism
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

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