In vivo 4D imaging of POM anterior segment colonization. (A) 4D in vivo imaging of the AS conducted between 24–48 hpf using POM transgenic lines. Time stamp hours:minutes. Scale bar = 50 μm. (B) Individual cell tracking for each POM transgenic line reveals migratory patterns during early AS colonization. Solid spheres indicate terminal end of track. (C–E) Cell tracking measurements of average migratory velocity (ANOVA p < 0.0001), total migration distance (ANOVA p < 0.0001), and migratory displacement within the AS (ANOVA p < 0.0001).
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