ZFIN ID: ZDB-FIG-200521-11
Caetano-Lopes et al., 2020 - Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish. Development (Cambridge, England)   147(2) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Adult

Fig. 1 Divergent expression of csf1ra and csf1rb during zebrafish early development and in scales of the adult zebrafish.

Whole-mount in situ hybridization of csf1ra (A) and csf1rb (B). (A) csf1ra is expressed in cells of the pigment and hematopoietic lineages at 2 dpf; inset shows the trunk in different focal plane with pigment cells precursors (arrowheads) and hematopoietic cells (arrows). (B) In contrast, csf1rb is expressed at only low levels in the heart (arrow) at 2 dpf. (C) Expression of Tg[cstk:DsRed] in osteoclasts localizes at areas of remodeling, as shown by increased calcein staining (blue) at the periphery of the remodeled pit. (D,E) Analysis of expression of csf1r paralogues in isolated cells and from adult skeletal tissues. (D) Expression of csfr1a and csf1rb in isolated adult osteoblasts (sp7+ cells) and osteoclasts (ctsk+ cells) from wild-type zebrafish. (E) Detection of both paralogues in isolated tissues [fin, teeth and vertebra (vert)].

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
csf1ra AB standard conditions Long-pec hematopoietic cell ISH
Long-pec migratory neural crest ISH
Adult Not Detected osteoblast RTPCR
Adult osteoclast RTPCR
mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast RTPCR
Adult fin osteoclast RTPCR
Adult vertebra osteoclast RTPCR
csf1rb AB standard conditions Long-pec heart ISH
Long-pec Not Detected hematopoietic cell ISH
Long-pec Not Detected migratory neural crest ISH
Adult Not Detected osteoblast RTPCR
Adult osteoclast RTPCR
mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast RTPCR
Adult fin osteoclast RTPCR
Adult vertebra osteoclast RTPCR
ctsk AB standard conditions Adult Not Detected osteoblast RTPCR
Adult osteoclast RTPCR
DsRed mh201Tg standard conditions Days 14-20 osteoclast IFL
Adult ceratobranchial 5 tooth osteoclast IFL
Adult fin osteoclast IFL
Adult vertebra osteoclast IFL
sp7 AB standard conditions Adult osteoblast RTPCR
Adult Not Detected osteoclast RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development