ZFIN ID: ZDB-FIG-200514-9
Koth et al., 2020 - Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration. Development (Cambridge, England)   147(8) Full text @ Development
Anatomical Terms:
Stage: Adult

Fig. 1 Runx1-Citrine becomes strongly expressed in the heart after cryo-injury. (A-D″) Immunohistochemistry for Runx1-Citrine (GFP antibody) and the myocardial marker MF20 at different time points after cryo-injury. (A,A′) Citrine expression in the uninjured hearts was confined to a small number of cells scattered around the heart (arrowheads). (B-B″) At 1 dpci, the epicardium was Citrine positive (arrowheads). Bright blood cells were visible within the wound and there was dim expression of Citrine overlapping with MF20 (B″). (C-C″) At 3 dpci, the epicardium, endocardium (arrowheads) and other wound cells were positive for Citrine. In addition, the myocardium in the border zone next to the wound was highly Citrine positive (C″). (D-D″) Expression of Citrine diminishes at 14 dpci but is still visible, especially in the myocardium (D″). (E,E′) In situ hybridisation for itga2b with immunohistochemistry for Runx1-Citrine and nuclear marker DAPI. Arrowheads indicate overlap of Runx1-Citrine with itg2b mRNA, indicating that thrombocytes are positive for Runx1-Citrine. a, atrium; ba, bulbus arteriosus; dpci, days post cryo-injury; en, endocardium; ep, epicardium; v, ventricle; w, wound. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine ox1Tg control Adult blood IHC
ox1Tg cold damage: cardiac ventricle Adult blood IHC
Adult epicardium IHC
Adult regenerating tissue cardiac muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC ox1Tg control Adult myocardium
IHC ox1Tg cold damage: cardiac ventricle Adult myocardium
Phenotype Details No data available
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