ZFIN ID: ZDB-FIG-200514-9
Koth et al., 2020 - Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration. Development (Cambridge, England)   147(8) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Fig. 1 Runx1-Citrine becomes strongly expressed in the heart after cryo-injury. (A-D″) Immunohistochemistry for Runx1-Citrine (GFP antibody) and the myocardial marker MF20 at different time points after cryo-injury. (A,A′) Citrine expression in the uninjured hearts was confined to a small number of cells scattered around the heart (arrowheads). (B-B″) At 1 dpci, the epicardium was Citrine positive (arrowheads). Bright blood cells were visible within the wound and there was dim expression of Citrine overlapping with MF20 (B″). (C-C″) At 3 dpci, the epicardium, endocardium (arrowheads) and other wound cells were positive for Citrine. In addition, the myocardium in the border zone next to the wound was highly Citrine positive (C″). (D-D″) Expression of Citrine diminishes at 14 dpci but is still visible, especially in the myocardium (D″). (E,E′) In situ hybridisation for itga2b with immunohistochemistry for Runx1-Citrine and nuclear marker DAPI. Arrowheads indicate overlap of Runx1-Citrine with itg2b mRNA, indicating that thrombocytes are positive for Runx1-Citrine. a, atrium; ba, bulbus arteriosus; dpci, days post cryo-injury; en, endocardium; ep, epicardium; v, ventricle; w, wound. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine ox1Tg control Adult blood IHC
ox1Tg cold damage: cardiac ventricle Adult blood IHC
Adult epicardium IHC
Adult regenerating tissue cardiac muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC ox1Tg control Adult myocardium
IHC ox1Tg cold damage: cardiac ventricle Adult myocardium
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development