ZFIN ID: ZDB-FIG-200514-14
Koth et al., 2020 - Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration. Development (Cambridge, England)   147(8) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 8 Myh11-positive endocardial cells and thrombocytes retain their double identity. (A-B′) In situ hybridisation for itga2b combined with immunohistochemistry for Citrine and Myh11. Arrowheads indicate Myh11-positive itga2b-positive thrombocytes present in the wild-type wound that are largely missing in the runx1 mutant wound at 7 dpci. (C-D′) In situ hybridisation for itga2b combined with immunohistochemistry for Myh11 with nuclear Dapi staining. Both the endocardium (inset) and thrombocytes (arrowheads) still express Myh11 in the wild-type wound at 14 dpci, while being absent in the mutant wound. v, ventricle; w, wound. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
itga2b ox1Tg cold damage: cardiac ventricle Adult endocardium ISH
Adult thrombocyte ISH
runx1hg1/hg1; ox1Tg cold damage: cardiac ventricle Adult endocardium ISH
Adult Not Detected thrombocyte ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-myh11 IHC ox1Tg cold damage: cardiac ventricle Adult endocardium
IHC Adult thrombocyte
IHC runx1hg1/hg1; ox1Tg cold damage: cardiac ventricle Adult endocardium
Phenotype Details
Fish Conditions Stage Phenotype
runx1hg1/hg1; ox1Tg cold damage: cardiac ventricle Adult endocardium itga2b expression decreased distribution, abnormal
Adult endocardium Ab2-myh11 labeling decreased distribution, abnormal
Adult thrombocyte itga2b expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development