ZFIN ID: ZDB-FIG-200514-12
Koth et al., 2020 - Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration. Development (Cambridge, England)   147(8) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 4 Runx1-Citrine positive endocardial cells appear in the wound after injury. (A-D,F,G) Immunohistochemistry analysis for Citrine- and mCherry-positive cells in the Tg(BAC-runx1P2:Citrine;kdrl:Hsa.HRAS-mCherry) line. (A,A′) The wound at 1 dpci, with the box highlighting the flat and weakly Citrine- and mCherry-positive endocardial cells in the wound (arrowhead). (B-C′) Clearly visible and round Citrine- and mCherry-positive cells in the wound at 3 dpci (B,B′, arrowheads), but not further away from the wound (C,C′). (D) At 14 dpci, not many double-positive cells are visible anymore. (E) Quantification of the number of Citrine and mCherry double-positive cells in and away from the wound, n=4, two-way ANOVA with Tukey's test. *P<0.05, **P<0.01 and ***P<0.001. Box extends from the 25th to 75th percentiles and whiskers indicate minimum to maximum with all data points shown. (F,G) runx1 mutant wounds have a reduced number of double-positive cells (arrowheads). en, endocardium; v, ventricle; w, wound. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine ox1Tg; s896Tg control Adult endocardium IHC
ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium IHC
runx1hg1/hg1; ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium IHC
mCherry ox1Tg; s896Tg control Adult endocardium IHC
ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium IHC
runx1hg1/hg1; ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium Citrine expression increased distribution, abnormal
Adult endocardium mCherry expression increased distribution, abnormal
runx1hg1/hg1; ox1Tg; s896Tg cold damage: cardiac ventricle Adult endocardium Citrine expression decreased distribution, abnormal
Adult endocardium mCherry expression decreased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development