Runx1-Citrine positive endocardial cells appear in the wound after injury. (A-D,F,G) Immunohistochemistry analysis for Citrine- and mCherry-positive cells in the Tg(BAC-runx1P2:Citrine;kdrl:Hsa.HRAS-mCherry) line. (A,A′) The wound at 1 dpci, with the box highlighting the flat and weakly Citrine- and mCherry-positive endocardial cells in the wound (arrowhead). (B-C′) Clearly visible and round Citrine- and mCherry-positive cells in the wound at 3 dpci (B,B′, arrowheads), but not further away from the wound (C,C′). (D) At 14 dpci, not many double-positive cells are visible anymore. (E) Quantification of the number of Citrine and mCherry double-positive cells in and away from the wound, n=4, two-way ANOVA with Tukey's test. *P<0.05, **P<0.01 and ***P<0.001. Box extends from the 25th to 75th percentiles and whiskers indicate minimum to maximum with all data points shown. (F,G) runx1 mutant wounds have a reduced number of double-positive cells (arrowheads). en, endocardium; v, ventricle; w, wound. Scale bars: 100 μm.
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