FIGURE

Figure 3

ID

ZDB-FIG-200413-3

Publication

Nguyen et al., 2020 - Anti-Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish

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Figure 3

Inhibitory effect of ethanol extract (EE) from leaves of C. cyrtophyllum Turcz on CuSO₄-stimulated ROS production in zebrafish larvae. The zebrafish were exposed to different concentrations of EE (5, 20, and 40 µg/mL) or 100 µM quercetin for 1 h and then exposed to 10 µM CuSO₄ for 20 min to induce oxidative stress. After 20 min, larvae were incubated with H2DCFDA (20 µg/mL) for 1 h. Fluorescent probes were used to detect and quantify oxidative stress by measuring the fluorescent intensity (FI) in larvae using Autovision software and a BD pathway 855 system (BD Biosciences). (A) Fluorescence intensity obtained from individual zebrafish larvae. The data are presented as medians for six different larvae, * p < 0.05 and ** p < 0.01 compared to the CuSO₄ alone Group. (B) Representative fluorescence micrographs of ROS production.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)