Figure 3

Figure 3

Inhibitory effect of ethanol extract (EE) from leaves of C. cyrtophyllum Turcz on CuSO₄-stimulated ROS production in zebrafish larvae. The zebrafish were exposed to different concentrations of EE (5, 20, and 40 µg/mL) or 100 µM quercetin for 1 h and then exposed to 10 µM CuSO₄ for 20 min to induce oxidative stress. After 20 min, larvae were incubated with H2DCFDA (20 µg/mL) for 1 h. Fluorescent probes were used to detect and quantify oxidative stress by measuring the fluorescent intensity (FI) in larvae using Autovision software and a BD pathway 855 system (BD Biosciences). (A) Fluorescence intensity obtained from individual zebrafish larvae. The data are presented as medians for six different larvae, * p < 0.05 and ** p < 0.01 compared to the CuSO₄ alone Group. (B) Representative fluorescence micrographs of ROS production.