Daily profiles of zCry1ab mRNA expression levels in the eye under different photoperiods. Zebrafish were entrained to 18L:6D extra-long-day (ELD; panel a), 14L:10D long-day (LD; panel b), 10L:14D short-day (SD; panel c) or 6L:18D extra-short-day (ESD; panel d) cycles. Eyes (n = 5, no mark; n = 4, +; n = 3, *) were collected to measure zCry1ab mRNA levels (see Methods for details) at the indicated time points from ZT1. Each mRNA level was estimated as a relative value to the geometric mean of mRNA levels of zβ-actin, zGapdh and zEf1α. Error bars represent ± SD. Light and dark periods are indicated in blue and grey, respectively. Data were analyzed by two-way ANOVA (Supplementary Table S58) and Tukey-Kramer post-hoc tests (Supplementary Tables S59–S63).
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