FIGURE

Fig. 1

ID

ZDB-FIG-200323-36

Publication

Gordon et al., 2019 - Fenestrae-associated protein Plvap regulates the rate of blood-borne proteins passage into the hypophysis

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Fig. 1

Zebrafish neurohypophyseal vasculature is permeable to blood-borne proteins. (A) Scheme describing an endogenous biosensor for real-time monitoring of vascular permeability. Vitamin D-binding protein (DBP) fused to EGFP, expressed in hepatocytes under a liver-specific promoter (l-fabp) and secreted into the general circulation, served as permeability biosensor. (B-F) Wholemounts of double transgenic Tg(l-fabp:DBP-EGFP;kdrl:mCherry-caax) zebrafish at different developmental stages demonstrating extravasation of DBP-EGFP in the pituitary (top) but not in the brain (bottom). A functional permeability boundary is established between the capillary loop and hypophyseal artery (dotted lines). Scale bars: 5 µm. (G,H) Wholemounts of double transgenic Tg(l-fabp:DBP-EGFP;kdrl:mCherry-caax) adult zebrafish hypophysis (G) and brain (H) vasculature. Scale bars: 20 µm. AMCtA, anterior (rostral) mesencephalic central artery; BCA, basal communicating artery; HypA, hypophyseal artery; HypV, hypophyseal vein; PCS, posterior (caudal) communicating segment.

Expression Data

Genes:	EGFP mCherry
Fish:	lri500Tg; y171Tg
Anatomical Terms:	anterior mesencephalic central artery blood plasma anterior mesencephalic central artery blood vessel endothelium basal communicating artery blood plasma basal communicating artery blood vessel endothelium brain extracellular space brain vasculature blood plasma brain vasculature blood vessel endothelium hypophyseal artery blood plasma hypophyseal artery blood vessel endothelium hypophyseal capillary blood plasma hypophyseal capillary blood vessel endothelium hypophyseal capillary fenestrated blood vessel endothelium hypophyseal vein blood plasma hypophyseal vein blood vessel endothelium hypophysis extracellular space posterior communicating artery blood plasma posterior communicating artery blood vessel endothelium
Stage Range:	Protruding-mouth to Adult

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Acknowledgments

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