FIGURE

Figure 5

ID

ZDB-FIG-200302-50

Publication

Otero-Asman et al., 2020 - The extracytoplasmic function sigma factor σ^VreI is active during infection and contributes to phosphate starvation-induced virulence of Pseudomonas aeruginosa

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Figure 5

σ^VreI activation during P. aeruginosa infection in zebrafish embryos. (A) Dorsal view of the head of a two days post-fertilization zebrafish embryo. The hindbrain ventricle where P. aeruginosa was injected is highlighted. (B) Confocal images of the head of embryos injected in the hindbrain ventricle with ∼2000 CFU of the P. aeruginosa PAO1 wild-type strain or its isogenic ΔvreI mutant bearing the σ^VreI-dependent pdtA::rfp transcriptional fusion (pMP0690mCherry plasmid, Table S1) (red channel) at 0 and 12 hpi. P. aeruginosa was grown in high Pi medium prior injection. Neutrophils expressing constitutively a green fluorescence protein (gfp) are also visualized (green channel). Images are representative of three independent experiments (N = 3).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; mpv17^a9/a9; i114Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa
Observed In:	hindbrain neutrophil chemotaxis
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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