ZFIN Image: Otero-Asman et al., 2020, Figure 5

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Figure 5

σ^VreI activation during P. aeruginosa infection in zebrafish embryos. (A) Dorsal view of the head of a two days post-fertilization zebrafish embryo. The hindbrain ventricle where P. aeruginosa was injected is highlighted. (B) Confocal images of the head of embryos injected in the hindbrain ventricle with ∼2000 CFU of the P. aeruginosa PAO1 wild-type strain or its isogenic ΔvreI mutant bearing the σ^VreI-dependent pdtA::rfp transcriptional fusion (pMP0690mCherry plasmid, Table S1) (red channel) at 0 and 12 hpi. P. aeruginosa was grown in high Pi medium prior injection. Neutrophils expressing constitutively a green fluorescence protein (gfp) are also visualized (green channel). Images are representative of three independent experiments (N = 3).

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Acknowledgments

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