IMAGE

Figure 5

ID: ZDB-IMAGE-200302-50
Publication: Otero-Asman et al., 2020 - The extracytoplasmic function sigma factor σ^VreI is active during infection and contributes to phosphate starvation-induced virulence of Pseudomonas aeruginosa
All Figures: Figures for Otero-Asman et al., 2020

Image

Figure Caption

Figure 5

σ^VreI activation during P. aeruginosa infection in zebrafish embryos. (A) Dorsal view of the head of a two days post-fertilization zebrafish embryo. The hindbrain ventricle where P. aeruginosa was injected is highlighted. (B) Confocal images of the head of embryos injected in the hindbrain ventricle with ∼2000 CFU of the P. aeruginosa PAO1 wild-type strain or its isogenic ΔvreI mutant bearing the σ^VreI-dependent pdtA::rfp transcriptional fusion (pMP0690mCherry plasmid, Table S1) (red channel) at 0 and 12 hpi. P. aeruginosa was grown in high Pi medium prior injection. Neutrophils expressing constitutively a green fluorescence protein (gfp) are also visualized (green channel). Images are representative of three independent experiments (N = 3).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.