FIGURE

Figure 6

ID

ZDB-FIG-200302-51

Publication

Otero-Asman et al., 2020 - The extracytoplasmic function sigma factor σ^VreI is active during infection and contributes to phosphate starvation-induced virulence of Pseudomonas aeruginosa

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Figure 6

Activation of σ^VreI upon interaction of P. aeruginosa with A549 eukaryotic cells. (A) Confocal images of P. aeruginosa-A549 co-cultures at 0 and 10 hpi. P. aeruginosa PAO1 wild-type strain and its isogenic ∆vreI mutant expressing a green fluorescent protein (gfp) constitutively (from the pBBRmEos3.1 plasmid, Table S1) (green channel) and containing the σ^VreI-dependent pdtA::rfp transcriptional fusion (pMP0690mCherry plasmid, Table S1) (red channel) were grown in high Pi medium and inoculated in A549-containing and A549-free cultures. A549 cells DNA was stained with DAPI (blue channel). Images are representative of three independent experiments (N = 3). (B) Quantification of the red fluorescence intensity observed in (A) was performed as described in Materials and Methods, and the total corrected cellular fluorescence (TCCF) is given. Data are means ± SD from three biological replicates (N = 3). P-value was calculated by unpaired two-tailed t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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