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<italic>Dstyk</italic> gene mutation leads to abnormal vesicle trafficking and dysregulated notochord vacuoles biogenesis.a Live confocal images of notochord for WT and dstyk mutant in bright-field and staining with MED at 36 hpf (top) and 4 dpf (bottom). b Live confocal images of notochord for WT and dstyk mutant dyed with Golgi tracker BODIPY TR Ceramide and LysoTracker Green at 7 dpf. Note that every notochord cell in the mutant was composed of one LysoTracker Green labeled vacuole (green) and at least one round cystic structure (white arrows) that can be labeled by Golgi tracker. c Confocal images of immunofluorescent staining of WT and dstyk mutants for Rab7a at 36 hpf in the background of Tg(β-actin:ras-GFP). d ISH of shh at 28 hpf (left) and 36 hpf (right) of WT and dstyk mutants. Magnified images in the bottom column. e ISH of cmn at 28 hpf of WT and dstyk mutants. f–h ISH of col8a1a (f), col9a1b (g) and col11a2 (h) at 36 hpf of WT and dstyk mutants. Magnified images in the bottom column. Scale bar represent 50 μm in (a–c), 200 μm in (d–h).
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