FIGURE

Fig. 3

ID

ZDB-FIG-200129-13

Publication

Sun et al., 2020 - Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway

Other Figures

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Fig. 3

Expression pattern of <italic>dstyk</italic> and knock out <italic>dstyk</italic> by CRISPR/Cas9.

aDstyk ISH at 1-cell/2-cell, 4-somite and 8-somite stage. b From 18-somite, 24 hpf, 38 hpf to 54 hpf, notochordal expression of dstyk was dynamically changed as detected by ISH. c The Cas9 target site for knockout dstyk gene worked in knock out F0 generation. d Top panel showed the DNA sequences for WT and two Cas9 mutant lines. TGG (red) is the PAM sequence. Deletion is represented by a red dashed line and insertion is highlighted in green. Bottom panel diagramed DSTYK protein structures of WT and predicted mutants. Arrows indicate amino acid at the mutation site. e Bright-field images showing the WT and two Cas9 mutant lines at 4 dpf. f Cas9 mutant lines showed similar scoliosis phenotypes with smt mutant. Scale bar represent 200 μm in (a and b), 400 μm in (e).

Expression Data

Gene:	dstyk
Fish:	AB
Anatomical Terms:	head notochord notochord posterior region whole organism
Stage Range:	2-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dstyk^{zf3306/zf3306} (AB) dstyk^{zf3307/zf3307} (AB)
Observed In:	somite trunk vertebral column whole organism
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.